Identification of Proteins Associated with the Formation of Oral Biofilms
Ver/
Descargar
(application/pdf: 3.399Mb)
(application/pdf: 3.399Mb)
Fecha
04- 01 -20Autor(es)
Millones Gómez, Pablo A
Metadatos
Mostrar el registro completo del ítemResumen
Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium
nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F.
nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via
amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated
with the biofilms and individual species were quantified using Western blotting. Results: The protein
profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional
electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were
determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was
present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the
formed biofilms; thus, HSP40 is an essential proteins for adhesion.
Palabras clave
Colecciones
El ítem tiene asociados los siguientes ficheros de licencia: